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Intraerythrocytic ring-shaped bodies morphologically compatible with small piroplasms were observed by LM in 59 57 true infections of the blood samples.

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This, along with the fact that T.

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Babesia canis, Babesia vogeli, B.

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Other authors have reported cases of T.

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At present, 12 piroplasm species have been reported in dogs worldwide but some of these have been only detected by molecular techniques [ 1 ].

Background

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In Europe, B.

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Fixed erythrocytes infected with Babesia microti were used as antigen.

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Among all piroplasm species reported in Europe, T.

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IFAT for anti-E.

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Historically, Babesia infection in dogs was identified according to the morphologic appearance of the parasite in the erythrocyte.

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Slides were examined by the same reader under a fluorescence microscope.

However, in most cases the travel history of the dogs was unknown.

The transmission vector of T.

Cardiosporidium cionae was used as an outgroup based on previous recommendations [ 37 ].

Positive sera were further tested in a serial dilution series from1:

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